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Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.
This new edition of the standard yeast identification and reference manual is the most up-to-date ever published. Over half of the volume is devoted to descriptions of the 678 currently recognised species, presented in a clear, easy to use layout and illustrated with over 1300 high quality photomicrographs. Readily usable keys and tables allow identification of all of the species described and a wealth of reference information broadens the scope of the book beyond identification. The book provides: - 678 species descriptions, with the results of 99 physiological tests displayed at a glance - Over 1300 high quality photomicrographs to accompany the descriptions, including 500 photomicrographs new to this edition - Nine identification keys, based on clearly defined groups of yeasts - Tables for identifying each species - A summary of the characteristics of 93 yeast genera - A list of nearly 4000 published yeast names, with provenances and synonyms - A list of specific epithets, with the genera to which they have belonged - General sections on yeast classification and laboratory methods - An extensive bibliography and a comprehensive glossary
Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biologyfrom metabolism and molecular genetics to cell division and differentiation. Thanks to its short generation time, ease of genetic manipulation, and suitability for high-throughput studies, yeast remains the focus of research in a vast number of laboratories worldwide.
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