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THE YEAST OF YERUSHALAIM. Jesus said that the Kingdom of God is like yeast. As yeast is worked into the dough and changes its structure positively, so the gospel is dispersed into societies and changes them from the inside. To mix the yeast of the gospel into societies, Jesus sent His disciples out to make disciples of all nations: "You shall receive power when the Holy Spirit has come upon you; and you shall be witnesses to Me in Jerusalem, and in all Judea and Samaria, and to the end of the earth" (Acts 1:8). Jerusalem is pronounced Yerushalaim in Hebrew. In this city Jesus was crucified, raised from the dead, and ascended to heaven, and to this city He will return. In Jerusalem, the Holy Spirit came into the hearts of believers, inspiring them to spread the gospel worldwide. The Acts and letters of the apostles show how the yeast spread throughout the Roman empire in the first century. When the yeast of Yerushalaim has done its work, Christ will return to renew heaven, earth, and Jerusalem.
Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.
Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biologyfrom metabolism and molecular genetics to cell division and differentiation. Thanks to its short generation time, ease of genetic manipulation, and suitability for high-throughput studies, yeast remains the focus of research in a vast number of laboratories worldwide.
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